Belgis. 2010, Production and Characterization of Cholesterol Biosensor with Immobilization Technique using Cholesterol Oxidase onto Polyaniline Membrane Conducting Chitosan. This script below supervising of Siti Wafiroh, S.Si, M.Si. and Dr.rer.nat. Ganden Supriyanto, M.Sc., Departement of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya.
Various methodes have been done to analyze cholesterol level, such as using amperometric methode, but the result is not maximal yet . Cholesterol biosensor with covalent immobilization techniques of cholesterol oxidase onto polyaniline membrane conducting chitosan, has been developed and characterized in an effort to develop a method of determination of cholesterol content. Before determining biosensor parameters validation such as linier range, detection limit, quantition limit, time response, sensitivity, precision, accuracy and stability of biosensor, pH optimum and maximum wavelength of standard solution of cholesterol were determined. The results of the optimization, biosensor has maximum wavelength of 650 nm and optimum pH is 7. Immobilization time for 24 hours. Biosensor response time of 8 minutes, and the biosensor can be used only once during the period of two weeks. Linier range of biosensor from 0,01 mg/ml to 2.50 mg/ml, with precision biosensor of 0.53 %, sensitivity of 0.304, detection limit of 0.00521 mg/ml and quantition limit of 0.0197 mg/ml. Accuracy of the biosensor was obtained 89.72 %.
Keywords: polyaniline, chitosan, biosensor, cholesterol oxidase